中国科学院大连化学物理研究所

1. Junfeng Ma, Jinxiang Liu, Liangliang Sun, Liang Gao, Zhen Liang, Lihua Zhang, Yukui Zhang, On-line Integrating Multiple Sample Pretreatment Steps Involving Denaturation, Reduction, and Digestion with μRPLC-ESI-MS/MS for High-Throughput Proteome Profiling, Anal. Chem., in press (2009)

2. Dingyin Tao, Guijie Zhu, Liangliang Sun, Junfeng Ma, Zhen Liang, Weibing Zhang, Lihua Zhang, Yukui Zhang, Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysis, Proteomics, 9, 2029-2036 (2009)

3. Junfeng Ma, Zhen Liang, Qiliang Deng, Xiaoqiang Qiao, Dingyin Tao, Lihua Zhang, Yukkui Zhang, Organic-Inorganic Hybrid Silica Monolith Based Immobilized Trypsin Reactor with High Enzymatic Activity, Anal. Chem., 80, 2049-2056 (2008)

4. Guijie Zhu, Huiming Yuan, Peng Zhao, Lihua Zhang, Zhen Liang, Weibing Zhang, Yukui Zhang, Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis, Electrophoresis, 18, 3578-2583 (2006)

5. Zhen Liang, Lihua Zhang, Jicheng Duan, Weibing Zhang, Chao Yan, Yukui Zhang, On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry, Electrophoresis, 26,1398-1405 (2005)

Research Interest:

High efficient separation and characterization of complex samples Selected Projects Undertaken:

1) National Basic Research Program of China (S973 project), 2012CB910600, Research on Novel Methods and Related Techniques for Protein Quantitation, 2012.01-2016.08, 27.5 million

2) Subproject of The Major Program of National Natural Science Foundation, 21190043, Novel aptamer based affinity enrichment and separation methods, 2012.01-2016.12, 3.5 million RMB

3) National Natural Science Foundation for Equipment, 21027002, Research and development on multilevel protein sample pretreatment, 2011.01-2013.12, 2 million RMB

4) Knowledge Innovation Program of Chinese Academy of Sciences, K2010A12, the application of novel technologies and methods for proteome separation and identification in the early detection of tumor metastatis, 2010.07-2012.06, 0.8 million RMB

Time	Honors and Awards
2013	"Young and middle-aged leading scientists, engineers and innovators" of Ministry of Science and Technology
2012	Second Prize of National Natural Science (rank second)
2011	First Prize of Natural Science in Liaoning Province (rank second)
2004	First Prize of Natural Science in Liaoning Province (rank second)

Selected papers:

1. Bo Jiang, Yu Liang, Qi Wu, Hao Jiang, Kaiguang Yang, Lihua Zhang*, Zhen Liang, Xiaojun Peng, Yukui Zhang, New GO-PEI-Au-L-Cys ZIC-HILIC, composites: synthesie and selective enrichment of glycopeptides, Nanoscale, 2014, 6: 5616-5619.

2. Jianxi Liu, Yanyan Qu, Kaiguang Yang, Qi Wu, Yichu Shan, Lihua Zhang*, Zhen Liang, Yukui Zhang, Monodisperse Boronate Polymeric Particles Synthesized by a Precipitation Polymerization Strategy: Particle Formation and Glycoprotein Response from the Standpoint of the Flory-Huggins Model, Acs Applied Materials & Interfaces, 2014, 6: 2059-2066.

3. Qun Zhao, Yu Liang, Huiming Yuan, Zhigang Sui, Qi Wu, Zhen Liang, Lihua Zhang*, Yukui Zhang, Biphasic Microreactor for Efficient Membrane Protein Pretreatment with a Combination of Formic Acid Assisted Solubilization, On-Column pH Adjustment, Reduction, Alkylation, and Tryptic Digestion, Analytical Chemistry, 2013, 85, 8507-8512.

4. Yuan Zhou, Yichu Shan, Qi Wu, Shen Zhang, Lihua Zhang*, Yukui Zhang, Mass Defect-Based Pseudo-Isobaric Dimethyl Labeling for Proteome Quantification, Analytical Chemistry, 2013, 85, 10658-10663.

5. Qun Zhao, Yu Liang, Huiming Yuan, Zhigang Sui, Qi Wu, Zhen Liang, Lihua Zhang*, Yukui Zhang, Biphasic Microreactor for Efficient Membrane Protein Pretreatment with a Combination of Formic Acid Assisted Solubilization, On-Column pH Adjustment, Reduction, Alkylation, and Tryptic Digestion, Analytical Chemistry, 2013, 85, 8507-8512.

6. Yuan Zhou, Yichu Shan, Qi Wu, Shen Zhang, Lihua Zhang*, Yukui Zhang, Mass Defect-Based Pseudo-Isobaric Dimethyl Labeling for Proteome Quantification, Analytical Chemistry, 2013, 85, 10658-10663.

7. Liyuan Zhang, Qun Zhao, Zhen Liang, Yang Kaiguang, Sun Liangliang, Lihua Zhang*, Yukui Zhang, Synthesis of adenosine functionalized metal immobilized magnetic nanoparticles for highly selective and sensitive enrichment of phosphopeptides, Chemical Communications, 2012, 48(50): 6274-6276.

8. Yanan Qu, Jianxi Liu, Kaiguang Yang, Zhen Liang, Lihua Zhang*, Yukui Zhang, Boronic Acid Functionalized Core-Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment, Chemistry-a European Journal, 2012, 18(29): 9056-9062.

9. Huiming Yuan, Yuan Zhou, Simin Xia, Lihua Zhang*, Xiaodan Zhang, Qi Wu, Zhen Liang, Yukui Zhang, Integrated Platform for Proteome Profiling with Combination of Microreversed Phase Based Protein and Peptide Separation via Online Solvent Exchange and Protein Digestion, Analytical Chemistry, 2012, 84(11): 5124-5132.

10. Junfeng Ma, Lihua Zhang*, Zhen Liang, Yichu Shan, Yukui Zhang, Immobilized enzyme reactors in proteomics, Trac-Trends in Analytical Chemistry, 2011, 30(5), 691-702.

11. Yanyan Qu, Simin Xia, Huiming Yuan, Qi Wu, Man Li, Lijuan Zou, Lihua Zhang*, Zhen Liang, Yukui Zhang, Integrated Sample Pretreatment System for N-Linked Glycosylation Site Profiling with Combination of Hydrophilic Interaction Chromatography and PNGase F Immobilized Enzymatic Reactor via a Strong Cation Exchange Precolumn, Analytical Chemistry, 2011, 83(19), 7457-7463.

12. Jinxiang Liu, Kaiguang Yang, Qiliang Deng, Qinran Li, Lihua Zhang*, Zhen Liang, Yukui Zhang, Preparation of a new type of affinity materials combining metal coordination with molecular imprinting, Chemical Communications, 2011, 47(13), 3969-3971.

13. Liangliang Sun, Jun Ma, Xiaoqiang Sun, Dingyin Tao, Yanyan Zhang, Yichu Shan, Zhen Liang, Lihua Zhang*, Yukui Zhang, Intergrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion, Analytical Chemistry, 2010, 82(6), 2574-2579.

14. Junfeng Ma, Chunyan Hou, Liangliang Sun, Dingyin Tao, Yanyan Zhang, Yichu Shan, Zhen Liang, Lihua Zhang* , Ling Yang, Yukui Zhang, Coupling Formic Acid Assisted Solubilization and online Immobi-lized Pepsin Microreactor Digestion with SCX-μPRLC-ESI-MS/MS for Integral Membrane Proteome Analysis, Analytical Chemistry, 2010, 82, 9622-9625.

Transferred patents :

1. Lihua Zhang (the first inventor) et al., A kind of capillary monolithic column and its applications in the immobilization of trypsin, 2013.5, China, ZL201010522957.4

2. Lihua Zhang (the first inventor) et al., A type of method for the preparation of porous monolithic material based immobilized enzyme microreactor,2012.3, China, ZL200810010347.9

3. Lihua Zhang (the first inventor) et al., Sample preprocessing device and its preparation for protein collection and enzymatic digestion, 2012.4, China, ZL200710159293.8

4. Lihua Zhang (the first inventor) et al., the device for the preparation of capillary electrospray interface used in Liquid chromatography-MS, 2012.5, China, ZL200710012718.2

5. Lihua Zhang (the first inventor) et al., the method and device for the online injection of large volume sample in microflow liquid chromatography, 2011.8, China, ZL200610134978.2

6. Lihua Zhang (the first inventor) et al., a type of multi-channel electrospray ion source for coupling of micro liquid separation system and mass spectrometry, 2012.4, China, ZL200610134979.7

7. Lihua Zhang (the first inventor) et al., System for the online desalting, enrichment and coupling of double split flow nanoliter scale liquid chromatography with mass spectrometry, 2011.4, China, ZL200610134981.4

张丽华 研究员;杰青

张丽华研究员;杰青